{"id":104280,"date":"2026-05-02T06:49:10","date_gmt":"2026-05-02T04:49:10","guid":{"rendered":"https:\/\/medqmedicalsuppliers.co.za\/?post_type=product&#038;p=104280"},"modified":"2026-05-02T06:49:16","modified_gmt":"2026-05-02T04:49:16","slug":"m-fc-agar-500g","status":"publish","type":"product","link":"https:\/\/medqmedicalsuppliers.co.za\/?product=m-fc-agar-500g","title":{"rendered":"M-FC Agar 500g"},"content":{"rendered":"<p>M-FC Agar 500g<\/p>\n<p>m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.<\/p>\n<p>Many standard method membrane filtration procedures recommend m-FC media for testing water.<br \/> The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 \ufffd\ufffd 0.5\ufffd\ufffdC in the fecal coliform procedure and other tests.<br \/> The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods.<br \/> The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.<br \/> Specifications<br \/> Brand Neogen\ufffd\ufffd Culture Media<br \/> Platform Dehydrated Culture Media<br \/> Mass 5 kg<br \/> Formula<br \/> Formula Liter<br \/> Enzymatic Digest of Casein 10.0 g\/L<br \/> Enzymatic Digest of Animal Tissue 5.0 g\/L<br \/> Yeast Extract 3.0 g\/L<br \/> Sodium Chloride 5.0 g\/L<br \/> Lactose 12.5 g\/L<br \/> Bile Salts 1.5 g\/L<br \/> Aniline Blue 0.1 g\/L<br \/> Agar 15.0 g\/L<br \/> Grams per Liter 52.00<br \/> Liters per 500 g 10.00<br \/> Preparation<br \/> Suspend 52 grams of the medium in one liter of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.<br \/> If necessary, adjust pH to 7.4 with 1N HCl.<br \/> Heat with frequent agitation and boil for one minute to completely dissolve the medium.<br \/> Cool to 45-50\ufffd\ufffdC and pour plates.<br \/> DO NOT AUTOCLAVE.<br \/> Previous Item Number Product was previously known as Acumedia Product: 7397 m FC Agar<br \/> Package Weight 14.00 lb<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>mFC Agar is used <b>for the detection and enumeration of faecal coliforms using membrane filter technique<\/b>.<\/p>\n<p>This medium is recommended by APHA. Store prepared media below 8\ufffd\ufffdC, protected from direct light.<\/p>\n<p>M-FC Agar 500g<\/p>\n","protected":false},"excerpt":{"rendered":"<p>M-FC Agar 500g<\/p>\n<p>m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans.<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment.<\/p>\n<p>Many standard method membrane filtration procedures recommend m-FC media for testing water.<br \/> The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 \ufffd\ufffd 0.5\ufffd\ufffdC in the fecal coliform procedure and other tests.<br \/> The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods.<br \/> The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.<br \/> Specifications<br \/> Brand Neogen\ufffd\ufffd Culture Media<br \/> Platform Dehydrated Culture Media<br \/> Mass 5 kg<br \/> Formula<br \/> Formula Liter<br \/> Enzymatic Digest of Casein 10.0 g\/L<br \/> Enzymatic Digest of Animal Tissue 5.0 g\/L<br \/> Yeast Extract 3.0 g\/L<br \/> Sodium Chloride 5.0 g\/L<br \/> Lactose 12.5 g\/L<br \/> Bile Salts 1.5 g\/L<br \/> Aniline Blue 0.1 g\/L<br \/> Agar 15.0 g\/L<br \/> Grams per Liter 52.00<br \/> Liters per 500 g 10.00<br \/> Preparation<br \/> Suspend 52 grams of the medium in one liter of purified water containing 10 mL of 1% Rosolic Acid in 0.2 N NaOH.<br \/> If necessary, adjust pH to 7.4 with 1N HCl.<br \/> Heat with frequent agitation and boil for one minute to completely dissolve the medium.<br \/> Cool to 45-50\ufffd\ufffdC and pour plates.<br \/> DO NOT AUTOCLAVE.<br \/> Previous Item Number Product was previously known as Acumedia Product: 7397 m FC Agar<br \/> Package Weight 14.00 lb<\/p>\n<p>M-FC Agar 500g<\/p>\n<p>mFC Agar is used <b>for the detection and enumeration of faecal coliforms using membrane filter technique<\/b>.<\/p>\n<p>This medium is recommended by APHA. Store prepared media below 8\ufffd\ufffdC, protected from direct light.<\/p>\n<p>M-FC Agar 500g<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"content-type":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[7056],"product_tag":[39625],"class_list":["post-104280","product","type-product","status-publish","product_cat-laboratory-chemicals-certified-reference-material","product_tag-m-fc-agar-500g","desktop-align-center","tablet-align-center","mobile-align-center","first","instock","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=\/wp\/v2\/product\/104280","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=\/wp\/v2\/product"}],"about":[{"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=\/wp\/v2\/types\/product"}],"wp:attachment":[{"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=104280"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_brand&post=104280"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_cat&post=104280"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/medqmedicalsuppliers.co.za\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_tag&post=104280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}